EnzIm2Flow Publications
Welcome to EnzIm2Flow's publications section. Here you will find a collection of our research papers and publications. Our work is focused on advancing the field of enzyme engineering and two-phase flow chemistry for sustainable chemical processes.
Immobilization of Oleate Hydratase on Solid Supports
Oleate hydratase from Rhodococcus erythropolis was immobilized on solid supports via anionic interaction. The immobilized enzyme was applied for synthesis of 10-hydroxystearic acid achieving product titer of 19 g L−1 at a very high TON of 19,000. Recycling of the biocatalyst for up to ten cycles was possible.
Novel oleate hydratases and potential biotechnological applications
In this paper we review the relevant consensus on the enzyme properties, including the limitations and issues with the reported data, the current understanding of the structure and mechanism of this enzyme, and the latest developments and outlooks on biocatalysis and biotechnological applications.
Exploring the abundance of oleate hydratases in the genus Rhodococcus—discovery of novel enzymes with complementary substrate scope
Oleate hydratases from two Rhodococcus strains (HFam 2 and 3) were heterologously expressed in Escherichia coli and their substrate scope investigated. Here, both enzymes showed a complementary behaviour towards sterically demanding and multiple unsaturated fatty acids. Furthermore, this study includes the characterisation of the newly discovered Rhodococcus pyridinivorans Ohy.
Immobilisation and flow chemistry: tools for implementing biocatalysis
In this feature article, we aim to point out important metrics for successful enzyme immobilisation and for reporting flow biocatalytic processes. Relevant examples of immobilised enzymes used in flow systems in organic, biphasic and aqueous systems are discussed. Finally, we describe recent developments to address the cofactor recycling hurdle.