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EnzIm2Flow - Oleate hydratase: engineering targeted enzyme immobilization for optimal conversion and product recovery using two-phase flow chemistry

EnzIm2Flow is a scientific research project centered around the enzyme oleate hydratase. We are dedicated to engineering targeted enzyme immobilization for optimal conversion and product recovery using two-phase flow chemistry.

Laboratory Pipette and Vials

About Us

EnzIm2Flow is at the forefront of sustainable chemical industry research, focusing on the conversion of renewable resources like vegetable oils into valuable compounds. Our team is committed to developing innovative solutions for industrial challenges, with a particular emphasis on the application of oleate hydratases. We are dedicated to utilizing controlled and optimized continuous flow processes, enhancing enzyme stability and efficiency through immobilization techniques.

Latest Publications

A Comparative Biochemical Study of Oleate Hydratases

Four oleate hydratases (Ohys) from diverse homologous families (HFams) were characterized side-by-side in terms of expression levels, oligomeric state, flavin adenine dinucleotide (FAD) affinity, thermostability, and activity. A general favorable effect of the reduction of FAD to FADH2 for Ohy activity was demonstrated, with (R)-10-hydroxystearic acid (10-HSA) yield increasing up to 12-fold.

Pipette and test plate

Contact

Department of Biotechnology

Delft University of Technology

Van der Maasweg 9, 2629HZ, Delft

The Netherlands

p.l.hagedoorn[at]tudelft.nl

+31-(0)15-2782334

© 2025 by P.L. Hagedoorn. Powered and secured by Wix

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